Hotspot

rbcL-psaI

LSC · 55874-57459 · 1586 bp

Back to Calamagrostis

Pi 0.00389

MarkerSeek score 37.60

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1586
variable_sites: 11
pi: 0.00389
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GATCATATCCACGCCGGTAC	CACGACTCCCTGTGGAAAAA	55.0	50.0	58.0	58.0	2697-2774 bp	1.0	80.0
rbcL-psaI_p2	ATATCCACGCCGGTACAGTA	CACGACTCCCTGTGGAAAAA	50.0	50.0	58.0	58.0	2693-2770 bp	1.0	80.0
rbcL-psaI_p3	GATCATATCCACGCCGGTAC	CCAAGATAACTGGAAGCCCC	55.0	55.0	58.0	57.9	2636-2713 bp	1.0	79.9
rbcL-psaI_p4	CATAACTGGGGGATTCACCG	CACGACTCCCTGTGGAAAAA	55.0	50.0	58.0	58.0	2860-2937 bp	1.0	79.8
rbcL-psaI_p5	ATATCCACGCCGGTACAGTA	CCAAGATAACTGGAAGCCCC	50.0	55.0	58.0	57.9	2632-2709 bp	1.0	79.8