Hotspot

trnT-GGU-trnE-UUC

LSC · 14894-15428 · 535 bp

Back to Calamagrostis

Pi 0.00436

MarkerSeek score 50.90

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 535
variable_sites: 4
pi: 0.00436
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	AGCCCTTTTAACTCAGTGGT	AAATACTCCCTACCCCCAGG	45.0	55.0	56.7	57.8	691 bp	1.0	68.0
trnT-GGU-trnE-UUC_p2	AGGCATAAGTCATCGGTTCA	AAATACTCCCTACCCCCAGG	45.0	55.0	56.6	57.8	654 bp	1.0	67.9
trnT-GGU-trnE-UUC_p3	AGCCCTTTTAACTCAGTGGT	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.7	58.3	656 bp	1.0	67.6
trnT-GGU-trnE-UUC_p4	AGCCCTTTTAACTCAGTGGT	TGTCCTGAACCACTAGACGA	45.0	50.0	56.7	57.7	635 bp	1.0	67.5
trnT-GGU-trnE-UUC_p5	AGGCATAAGTCATCGGTTCA	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.6	58.3	619 bp	1.0	67.4