Hotspot

trnfM-CAU-trnG-UCC

LSC · 12734-12837 · 104 bp

Back to Calamagrostis

Pi 0.0112

MarkerSeek score 53.40

Primer available yes

Label trnfM-CAU-trnG-UCC

Feature Metadata

label_name: trnfM-CAU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 104
variable_sites: 2
pi: 0.0112
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnG-UCC_p1	CCTCAAGGTTATGAGCCTCG	AGCCTTCCAAGCTAACGATG	55.0	50.0	57.8	58.0	192-195 bp	1.0	80.0
trnfM-CAU-trnG-UCC_p2	CCTCAAGGTTATGAGCCTCG	CCTAGCCTTCCAAGCTAACG	55.0	55.0	57.8	58.1	195-198 bp	1.0	79.7
trnfM-CAU-trnG-UCC_p3	CCTCGTGAGCTACCAAACTG	AGCCTTCCAAGCTAACGATG	55.0	50.0	58.3	58.0	177-180 bp	1.0	79.7
trnfM-CAU-trnG-UCC_p4	CCTCGTGAGCTACCAAACTG	CCTAGCCTTCCAAGCTAACG	55.0	55.0	58.3	58.1	180-183 bp	1.0	79.5
trnfM-CAU-trnG-UCC_p5	CCTCAAGGTTATGAGCCTCG	CCAACGTCGACTATAACCCC	55.0	55.0	57.8	57.8	213-216 bp	1.0	78.3