Hotspot

psbK-psbI

LSC · 6950-7351 · 402 bp

Back to Calamagrostis

Pi 0.00498

MarkerSeek score 39.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 402
variable_sites: 3
pi: 0.00498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGTGGATGTTATGCCTGTCA	TTCCGTCCTGGGTCATTAGA	50.0	50.0	58.0	57.8	573-574 bp	1.0	78.3
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCCGTCCTGGGTCATTAGA	50.0	50.0	57.9	57.8	524-525 bp	1.0	78.1
psbK-psbI_p3	CGTGGATGTTATGCCTGTCA	GAATTTTTACTCCTCGCGCC	50.0	50.0	58.0	57.5	598-599 bp	1.0	76.6
psbK-psbI_p4	CGTGGATGTTATGCCTGTCA	GGAATTTTTACTCCTCGCGC	50.0	50.0	58.0	57.5	599-600 bp	1.0	76.6
psbK-psbI_p5	CGTGGATGTTATGCCTGTCA	TCCGTCCTGGGTCATTAGAT	50.0	50.0	58.0	57.5	572-573 bp	1.0	76.5