Hotspot

rpl16

LSC · 85407-87554 · 2148 bp

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Pi 0.076

MarkerSeek score 83.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 2148
variable_sites: 262
pi: 0.076
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATTGGGCACTGCTTCTTTGA	TTCCCCTACAGACCATTCGT	45.0	50.0	58.0	58.1	2569-2690 bp	0.5	63.7
rpl16_p2	TGGGCACTGCTTCTTTGATT	TTCCCCTACAGACCATTCGT	45.0	50.0	58.0	58.1	2567-2688 bp	0.5	63.7
rpl16_p3	TTGGGCACTGCTTCTTTGAT	TTCCCCTACAGACCATTCGT	45.0	50.0	58.0	58.1	2568-2689 bp	0.5	63.7
rpl16_p4	CTCGAGCACCGCTATTATCC	TTCCCCTACAGACCATTCGT	55.0	50.0	58.0	58.1	2474-2595 bp	0.5	63.6
rpl16_p5	TCTCAATTCCCTGGCGATTG	TTCCCCTACAGACCATTCGT	50.0	50.0	58.0	58.1	2743-2864 bp	0.5	63.4