Hotspot

ndhB-trnL-CAA

IRa · 151719-152866 · 1148 bp

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Pi 0.0341

MarkerSeek score 85.80

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 1148
variable_sites: 70
pi: 0.0341
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	1208-1275 bp	1.0	88.0
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	1206-1273 bp	1.0	88.0
ndhB-trnL-CAA_p3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	1203-1270 bp	1.0	86.5
ndhB-trnL-CAA_p4	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	1207-1274 bp	1.0	85.9
ndhB-trnL-CAA_p5	TGCAATTGCTCAGGATACCC	TCGCGTGTCTACCATTTCAC	50.0	50.0	57.9	58.3	1205-1272 bp	1.0	85.7