Hotspot

ndhG-ndhI

SSC · 125549-126119 · 571 bp

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Pi 0.102

MarkerSeek score 73.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 571
variable_sites: 95
pi: 0.102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAGGCCCCCTAAAATAA	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	970-1185 bp	0.75	76.3
ndhG-ndhI_p2	TCCCAGGCCCCCTAAAATAA	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	969-1184 bp	0.75	76.3
ndhG-ndhI_p3	CAGCGAAGTAACCCCATCTC	AACAAACAGTACGAGCTGCA	55.0	45.0	58.1	58.1	1201-1416 bp	0.75	75.6
ndhG-ndhI_p4	CAGCGAAGTAACCCCATCTC	ACAAACAGTACGAGCTGCAA	55.0	45.0	58.1	58.1	1200-1415 bp	0.75	75.6
ndhG-ndhI_p5	TGTGCGGCAGCTACAAAATA	ACAAACAGTACGAGCTGCAA	45.0	45.0	58.2	58.1	1079-1294 bp	0.75	74.7