Hotspot

trnF-GAA-ndhJ

LSC · 51171-52553 · 1383 bp

Back to Caladium

Pi 0.105

MarkerSeek score 87.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 1383
variable_sites: 237
pi: 0.105
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 87.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ACGTAAGGACTATATTACCCCCA	50.0	43.5	58.1	57.9	1289-1889 bp	1.0	67.0
trnF-GAA-ndhJ_p2	AATGGTCGGGATAGCTCAGT	ACGTAAGGACTATATTACCCCCA	50.0	43.5	58.2	57.9	1332-1932 bp	1.0	65.7
trnF-GAA-ndhJ_p3	ATGGTCGGGATAGCTCAGTT	ACGTAAGGACTATATTACCCCCA	50.0	43.5	58.2	57.9	1331-1931 bp	1.0	65.7
trnF-GAA-ndhJ_p4	TCGGGATAGCTCAGTTGGTA	ACGTAAGGACTATATTACCCCCA	50.0	43.5	57.6	57.9	1327-1927 bp	1.0	63.8
trnF-GAA-ndhJ_p5	AATCCTCGTGTCACCAGTTC	ACGTAAGGACTATATTACCCCCA	50.0	43.5	57.5	57.9	1292-1892 bp	1.0	63.7