Hotspot

petN-psbM

LSC · 31028-32276 · 1249 bp

Back to Caladium

Pi 0.0125

MarkerSeek score 68.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1249
variable_sites: 28
pi: 0.0125
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTTATTCT	55.0	38.1	58.1	55.2	1353-1401 bp	1.0	52.7
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTTATTCT	55.0	38.1	58.1	55.2	1352-1400 bp	1.0	52.7
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTTATTCT	50.0	38.1	58.2	55.2	1411-1459 bp	1.0	52.3
petN-psbM_p4	ATGGGGAAGGAGTGGACTTT	TGCTACTGCACTGTTTATTCT	50.0	38.1	58.2	55.2	1350-1398 bp	1.0	51.7
petN-psbM_p5	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTTATTCTAGT	55.0	37.5	58.1	58.0	1352-1400 bp	1.0	50.4