Hotspot

ycf1

SSC · 123277-128589 · 5313 bp

Pi 0.00163

MarkerSeek score 47.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5313
variable_sites: 26
pi: 0.00163
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTGGTATGACCCACGGTTTC	TTGAGCCTGATACTCACAGT	50.0	45.0	57.8	55.9	80-234 bp	1.0	63.8
ycf1_p2	GGTATGACCCACGGTTTCAT	TTGAGCCTGATACTCACAGT	50.0	45.0	57.6	55.9	80-232 bp	1.0	62.0
ycf1_p3	TTGGTATGACCCACGGTTTC	ATTGAGCCTGATACTCACAGT	50.0	42.9	57.8	56.4	80-235 bp	1.0	60.3
ycf1_p4	GGTATGACCCACGGTTTCAT	ATTGAGCCTGATACTCACAGT	50.0	42.9	57.6	56.4	80-233 bp	1.0	58.5
ycf1_p5	ATTGGTATGACCCACGGTTT	TTGAGCCTGATACTCACAGT	45.0	45.0	57.1	55.9	80-235 bp	1.0	58.2