Hotspot

rpl16

LSC · 80766-82272 · 1507 bp

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Pi 0.00121
MarkerSeek score 48.50
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1507
variable_sites
4
pi
0.00121
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCTCTTCTTTTAATGCAAAGGACA ATTTCCATCAAAACTCCTTTG 33.3 33.3 56.8 52.5 80-1673 bp 1.0 41.7
rpl16_p2 CTCTTCTTTTAATGCAAAGGACA ATTTCCATCAAAACTCCTTTG 34.8 33.3 55.4 52.5 80-1672 bp 1.0 41.7
rpl16_p3 TCTCTTCTTTTAATGCAAAGGACAA ATTTCCATCAAAACTCCTTTG 32.0 33.3 57.4 52.5 80-1673 bp 1.0 41.7
rpl16_p4 TCTCTTCTTTTAATGCAAAGGACA AATTTCCATCAAAACTCCTTTG 33.3 31.8 56.8 53.4 80-1674 bp 1.0 41.6
rpl16_p5 TCTCTTCTTTTAATGCAAAGGACA AAATTTCCATCAAAACTCCTTTG 33.3 30.4 56.8 54.3 80-1675 bp 1.0 41.6