Hotspot

ndhF-rpl32

SSC · 112129-112917 · 789 bp

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Pi 0.00242
MarkerSeek score 48.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
789
variable_sites
7
pi
0.00242
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAATGAAAGGGA ACGATGTCCAATATCCCTTCC 47.6 47.6 57.6 57.2 80-928 bp 1.0 55.1
ndhF-rpl32_p2 TTGTTGCTGTCGGAAAAAGT ACGATGTCCAATATCCCTTCC 40.0 47.6 56.4 57.2 80-976 bp 1.0 53.8
ndhF-rpl32_p3 TGGAAGTGGAATGAAAGGGA ACGATGTCCAATATCCCTTCC 45.0 47.6 56.3 57.2 80-927 bp 1.0 53.3
ndhF-rpl32_p4 CTGGAAGTGGAATGAAAGGGA CGATGTCCAATATCCCTTCCT 47.6 47.6 57.6 56.9 80-927 bp 1.0 52.8
ndhF-rpl32_p5 CTGGAAGTGGAATGAAAGGGA AACGATGTCCAATATCCCTTCC 47.6 45.5 57.6 57.9 80-929 bp 1.0 52.6