Hotspot

petA-psbJ

LSC · 62061-63041 · 981 bp

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Pi 0.000651
MarkerSeek score 48.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
981
variable_sites
3
pi
0.000651
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC CAGGATTAGGTTCATCCCTGT 50.0 47.6 58.0 56.8 80-1632 bp 1.0 62.4
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC TCAGGATTAGGTTCATCCCTGT 50.0 45.5 58.0 58.3 80-1633 bp 1.0 62.1
petA-psbJ_p3 CATTACGTGTCCAAGGCCTT CAGGATTAGGTTCATCCCTGT 50.0 47.6 58.2 56.8 80-1120 bp 1.0 61.4
petA-psbJ_p4 CATTACGTGTCCAAGGCCTT TCAGGATTAGGTTCATCCCTGT 50.0 45.5 58.2 58.3 80-1121 bp 1.0 61.1
petA-psbJ_p5 ATCCATTACGTGTCCAAGGC TCAGGATTAGGTTCATCCCTG 50.0 47.6 58.0 56.5 80-1633 bp 1.0 60.2