Hotspot

ycf4-cemA

LSC · 59709-60181 · 473 bp

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Pi 0.00324
MarkerSeek score 48.20
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
473
variable_sites
5
pi
0.00324
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 AGGTCAAGGGGCTATTCCTT AGAGAGATCAACCAGGGCAA 50.0 50.0 58.0 58.3 80-663 bp 1.0 78.0
ycf4-cemA_p2 TTGGCTTACTTCTTGCGTGT AGAGAGATCAACCAGGGCAA 45.0 50.0 58.0 58.3 80-587 bp 1.0 77.9
ycf4-cemA_p3 AGGTCAAGGGGCTATTCCTT TCAACCAGGGCAAAAAGACT 50.0 45.0 58.0 57.5 80-656 bp 1.0 77.1
ycf4-cemA_p4 TTGGCTTACTTCTTGCGTGT TCAACCAGGGCAAAAAGACT 45.0 45.0 58.0 57.5 80-580 bp 1.0 77.0
ycf4-cemA_p5 AGGTCAAGGGGCTATTCCTT GAGAGATCAACCAGGGCAAA 50.0 50.0 58.0 57.5 80-662 bp 1.0 76.8