Hotspot

psbM-trnD-GUC

LSC · 28479-29490 · 1012 bp

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Pi 0.00225
MarkerSeek score 48.20
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1012
variable_sites
7
pi
0.00225
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGGAACGAGAATGAAGAGTGC TACCCGCTTGATTTCCATGG 47.6 50.0 58.0 57.9 80-1159 bp 1.0 72.0
psbM-trnD-GUC_p2 ACGAGAATGAAGAGTGCAGT TACCCGCTTGATTTCCATGG 45.0 50.0 56.9 57.9 80-1155 bp 1.0 71.3
psbM-trnD-GUC_p3 AGGAACGAGAATGAAGAGTGC CCGCTTGATTTCCATGGGAT 47.6 50.0 58.0 58.3 80-1156 bp 1.0 70.0
psbM-trnD-GUC_p4 AGGAACGAGAATGAAGAGTGC GCTACCCGCTTGATTTCCAT 47.6 50.0 58.0 58.3 80-1161 bp 1.0 69.8
psbM-trnD-GUC_p5 GGAACGAGAATGAAGAGTGCA TACCCGCTTGATTTCCATGG 47.6 50.0 58.3 57.9 80-1158 bp 1.0 69.8