Hotspot

rps16-trnQ-UUG

LSC · 6091-6543 · 453 bp

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Pi 0.00418
MarkerSeek score 50.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
453
variable_sites
7
pi
0.00418
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 522-1966 bp 1.0 79.4
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 533-1967 bp 1.0 79.1
rps16-trnQ-UUG_p3 ATAAAAGGCGGCGGATCAAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.2 58.1 567-1967 bp 1.0 78.7
rps16-trnQ-UUG_p4 CCACAGCAGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 543-1966 bp 1.0 77.4
rps16-trnQ-UUG_p5 TCCACAGCAGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 544-1967 bp 1.0 77.3