Hotspot

ycf1

SSC · 109746-115100 · 5355 bp

Back to Cajanus

Pi 0.0208

MarkerSeek score 52.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5355
variable_sites: 111
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCTAGACGTCGTCGAGAATT	AAACGGCTTCCATTATGGGT	50.0	45.0	57.5	57.5	257 bp	1.0	77.1
ycf1_p2	CAAGCAAAATACGCCGATCG	AAACGGCTTCCATTATGGGT	50.0	45.0	58.3	57.5	880-881 bp	1.0	75.2
ycf1_p3	AAGCAAAATACGCCGATCGA	AAACGGCTTCCATTATGGGT	45.0	45.0	58.4	57.5	879-880 bp	1.0	74.5
ycf1_p4	AGCAAAATACGCCGATCGAA	AAACGGCTTCCATTATGGGT	45.0	45.0	58.4	57.5	878-879 bp	1.0	74.5
ycf1_p5	TGGGCAGTGAAAAGAATGGT	AAACGGCTTCCATTATGGGT	45.0	45.0	57.6	57.5	798-799 bp	1.0	74.5