Hotspot

rps3-rps19

LSC · 83704-84220 · 517 bp

Back to Cajanus

Pi 0.0719
MarkerSeek score 81.60
Primer available yes
Label rps3-rps19

Feature Metadata

label_name
rps3-rps19
feature_type
igs
region
LSC
length_bp
517
variable_sites
24
pi
0.0719
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.65
markerseek_score
81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rps19_p1 CAAGTCTGAAACCAAGCGGA AACTTCCGAGGACATGCAAA 50.0 45.0 58.4 57.7 440-602 bp 1.0 82.3
rps3-rps19_p2 CAAGTCTGAAACCAAGCGGA ACTTCCGAGGACATGCAAAA 50.0 45.0 58.4 57.7 439-601 bp 1.0 82.3
rps3-rps19_p3 TGGTTGTGCAAACCAAACAG AAACTTCCGAGGACATGCAA 45.0 45.0 57.6 57.7 482-644 bp 1.0 82.2
rps3-rps19_p4 TGGTTGTGCAAACCAAACAG AACTTCCGAGGACATGCAAA 45.0 45.0 57.6 57.7 481-643 bp 1.0 82.2
rps3-rps19_p5 TGGTTGTGCAAACCAAACAG ACTTCCGAGGACATGCAAAA 45.0 45.0 57.6 57.7 480-642 bp 1.0 82.2