Hotspot

rps11-rpl36

LSC · 79245-79626 · 382 bp

Back to Cajanus

Pi 0.0896

MarkerSeek score 89.30

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 382
variable_sites: 30
pi: 0.0896
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 89.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	GAGTATGCTTACGCGAACCA	AGACGGGGGCGAATTATAGT	50.0	50.0	58.1	58.3	494-509 bp	1.0	85.8
rps11-rpl36_p2	GAGTATGCTTACGCGAACCA	AAAATAAGGGCCTCCGTTCG	50.0	50.0	58.1	58.3	545-560 bp	1.0	85.7
rps11-rpl36_p3	CGTCCATTTTTACGCGAACC	AGACGGGGGCGAATTATAGT	50.0	50.0	58.1	58.3	471-486 bp	1.0	85.7
rps11-rpl36_p4	CGTCCATTTTTACGCGAACC	AAAATAAGGGCCTCCGTTCG	50.0	50.0	58.1	58.3	522-537 bp	1.0	85.5
rps11-rpl36_p5	TCCATTTTTACGCGAACCCT	AAAATAAGGGCCTCCGTTCG	45.0	50.0	57.8	58.3	520-535 bp	1.0	85.0