Hotspot

rpoA-rps11

LSC · 78738-78827 · 90 bp

Back to Cajanus

Pi 0.0345

MarkerSeek score 75.40

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 90
variable_sites: 3
pi: 0.0345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	ACACTTCCATTGTAGTGTCCG	AAGAGATGCGGCATTAAGGG	47.6	50.0	58.0	58.0	259-262 bp	1.0	73.3
rpoA-rps11_p2	ACACTTCCATTGTAGTGTCCG	CGGCATTAAGGGCTATTCGT	47.6	50.0	58.0	58.1	251-254 bp	1.0	72.6
rpoA-rps11_p3	ACACTTCCATTGTAGTGTCCG	GATGCGGCATTAAGGGCTAT	47.6	50.0	58.0	58.2	255-258 bp	1.0	72.2
rpoA-rps11_p4	GGAGACAGAATAAAGCGTCCA	AAGAGATGCGGCATTAAGGG	47.6	50.0	57.8	58.0	315-318 bp	1.0	71.3
rpoA-rps11_p5	TGGAGACAGAATAAAGCGTCC	AAGAGATGCGGCATTAAGGG	47.6	50.0	57.8	58.0	316-319 bp	1.0	71.3