Hotspot

trnW(cca)-trnP(tgg)

LSC · 65990-66181 · 192 bp

Back to Cajanus

Pi 0.0417

MarkerSeek score 68.70

Primer available yes

Label trnW(cca)-trnP(tgg)

Feature Metadata

label_name: trnW(cca)-trnP(tgg)
feature_type: igs
region: LSC
length_bp: 192
variable_sites: 8
pi: 0.0417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW(cca)-trnP(tgg)_p1	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	50.0	50.0	57.9	58.1	259-263 bp	1.0	81.2
trnW(cca)-trnP(tgg)_p2	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.9	58.1	248-252 bp	1.0	81.2
trnW(cca)-trnP(tgg)_p3	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	50.0	50.0	57.9	58.1	246-250 bp	1.0	81.2
trnW(cca)-trnP(tgg)_p4	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	50.0	50.0	57.9	58.1	249-253 bp	1.0	81.2
trnW(cca)-trnP(tgg)_p5	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	50.0	50.0	57.9	58.1	260-264 bp	1.0	81.1