Hotspot

psbE-petL

LSC · 64156-65397 · 1242 bp

Back to Cajanus

Pi 0.0255

MarkerSeek score 71.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1242
variable_sites: 23
pi: 0.0255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	ACTCAGACCAATAAAAAGAGCT	50.0	36.4	57.8	55.4	1038-1347 bp	1.0	47.2
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	ACTCAGACCAATAAAAAGAGCT	55.0	36.4	58.2	55.4	1033-1342 bp	1.0	46.9
psbE-petL_p3	CCTGCAATGAATAGGGAGGG	ACTCAGACCAATAAAAAGAGCT	55.0	36.4	57.7	55.4	1112-1421 bp	1.0	46.1
psbE-petL_p4	TCTCCTGTGCTTCCAGACAT	ACTCAGACCAATAAAAAGAGCT	50.0	36.4	58.4	55.4	1031-1340 bp	1.0	45.7
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGTCGTATTTTACTCAGACCA	50.0	38.1	57.8	54.1	1049-1358 bp	1.0	45.5