Hotspot

psbD-trnT(ggt)

LSC · 29137-30296 · 1160 bp

Back to Cajanus

Pi 0.0237

MarkerSeek score 76.90

Primer available yes

Label psbD-trnT(ggt)

Feature Metadata

label_name: psbD-trnT(ggt)
feature_type: igs
region: LSC
length_bp: 1160
variable_sites: 26
pi: 0.0237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT(ggt)_p1	ATAGACCAGACCAACCCACA	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.0	58.1	1328-1425 bp	1.0	84.5
psbD-trnT(ggt)_p2	ATAGACCAGACCAACCCACA	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.0	58.1	1331-1428 bp	1.0	84.3
psbD-trnT(ggt)_p3	AGACCAACCCACAAAAACGA	GTGGTAGAGTAACGCCATGG	45.0	55.0	57.9	58.1	1321-1418 bp	1.0	83.7
psbD-trnT(ggt)_p4	AGACCAACCCACAAAAACGA	TCAGTGGTAGAGTAACGCCA	45.0	50.0	57.9	58.1	1324-1421 bp	1.0	83.6
psbD-trnT(ggt)_p5	TAGACCAGACCAACCCACAA	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1327-1424 bp	1.0	83.3