Hotspot

trnG(gcc)-psbZ

LSC · 25117-25861 · 745 bp

Back to Cajanus

Pi 0.0678
MarkerSeek score 75.40
Primer available yes
Label trnG(gcc)-psbZ

Feature Metadata

label_name
trnG(gcc)-psbZ
feature_type
igs
region
LSC
length_bp
745
variable_sites
32
pi
0.0678
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.63
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG(gcc)-psbZ_p1 CGTCTTCTCCTTGGCAAAGA CGAAGGTTGGTCGAGTAACA 50.0 50.0 57.8 57.6 677-892 bp 1.0 82.5
trnG(gcc)-psbZ_p2 CGTCTTCTCCTTGGCAAAGA CGAAGGTTGGTCGAGTAACAA 50.0 47.6 57.8 58.3 677-892 bp 1.0 75.9
trnG(gcc)-psbZ_p3 ACATAACTAAGTGGAGGCGG CGAAGGTTGGTCGAGTAACA 50.0 50.0 57.0 57.6 716-931 bp 1.0 75.8
trnG(gcc)-psbZ_p4 CGTCTTCTCCTTGGCAAAGA TGTTGTATTTGCTTCTCCCGA 50.0 42.9 57.8 57.6 695-910 bp 1.0 74.5
trnG(gcc)-psbZ_p5 CGGATAGCGGGAATCGAAC CGAAGGTTGGTCGAGTAACA 57.9 50.0 58.2 57.6 699-914 bp 1.0 74.2