Hotspot

trnK-UUU-rbcL

LSC · 4425-5475 · 1051 bp

Back to Cajanus

Pi 0.0509

MarkerSeek score 76.50

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 1051
variable_sites: 52
pi: 0.0509
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	ACCATTGAGTTAGCAACCC	AGTCCTGAATTAGCTGCTGC	47.4	50.0	55.1	58.0	1145-1198 bp	1.0	53.7
trnK-UUU-rbcL_p2	ACCATTGAGTTAGCAACCC	GAGGCTAGCAAATGGAGTCC	47.4	55.0	55.1	58.0	1160-1213 bp	1.0	53.6
trnK-UUU-rbcL_p3	ACCATTGAGTTAGCAACCC	GGCTAGCAAATGGAGTCCTG	47.4	55.0	55.1	58.3	1158-1211 bp	1.0	51.4
trnK-UUU-rbcL_p4	ACCATTGAGTTAGCAACCC	GTGAGGCTAGCAAATGGAGT	47.4	50.0	55.1	57.6	1162-1215 bp	1.0	50.7
trnK-UUU-rbcL_p5	ACCATTGAGTTAGCAACCC	AGGCTAGCAAATGGAGTCCT	47.4	50.0	55.1	58.4	1159-1212 bp	1.0	50.7