Hotspot

psbA-trnK-UUU

LSC · 1482-1762 · 281 bp

Back to Cajanus

Pi 0.037

MarkerSeek score 73.70

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 281
variable_sites: 10
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	GCTCTCGCGTCTCTCTAAAA	CGGCTTGGGGAGAGATTTTT	50.0	50.0	57.5	58.2	402-413 bp	1.0	78.5
psbA-trnK-UUU_p2	TACAGAAGCGACCCCATAGG	CGGCTTGGGGAGAGATTTTT	55.0	50.0	58.6	58.2	427-438 bp	1.0	77.9
psbA-trnK-UUU_p3	ATCCAGTTACAGAAGCGACC	CGGCTTGGGGAGAGATTTTT	50.0	50.0	57.3	58.2	434-445 bp	1.0	77.1
psbA-trnK-UUU_p4	AGTTACAGAAGCGACCCCAT	CGGCTTGGGGAGAGATTTTT	50.0	50.0	58.7	58.2	430-441 bp	1.0	76.8
psbA-trnK-UUU_p5	GCTCTCGCGTCTCTCTAAAA	CTTCATCCGACTAGTTCCGG	50.0	55.0	57.5	57.5	341-352 bp	1.0	76.5