Hotspot

trnH(gtg)-psbA

LSC · 92-419 · 328 bp

Back to Cajanus

Pi 0.0332

MarkerSeek score 69.90

Primer available yes

Label trnH(gtg)-psbA

Feature Metadata

label_name: trnH(gtg)-psbA
feature_type: igs
region: LSC
length_bp: 328
variable_sites: 8
pi: 0.0332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH(gtg)-psbA_p1	CTGCCTTAATCCACTTGGCT	CCGAGCTAACCTTGGTATGG	50.0	55.0	57.9	57.8	372-453 bp	1.0	82.5
trnH(gtg)-psbA_p2	ACTGCCTTAATCCACTTGGC	CCGAGCTAACCTTGGTATGG	50.0	55.0	58.2	57.8	373-454 bp	1.0	82.3
trnH(gtg)-psbA_p3	AATCCACTTGGCTACATCCG	CCGAGCTAACCTTGGTATGG	50.0	55.0	57.7	57.8	365-446 bp	1.0	81.0
trnH(gtg)-psbA_p4	CGCATGGTGGATTCACAATC	CCGAGCTAACCTTGGTATGG	50.0	55.0	57.5	57.8	394-475 bp	1.0	79.7
trnH(gtg)-psbA_p5	ATATGAATGGGCGAACGACG	CCGAGCTAACCTTGGTATGG	50.0	55.0	58.5	57.8	508 bp	0.5	54.1