Hotspot

ycf1

SSC · 127376-133060 · 5685 bp

Back to Caiophora

Pi 0.00704

MarkerSeek score 51.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5685
variable_sites: 40
pi: 0.00704
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTACGACGTTGATTGGCTCT	TCTGTCTGAAACCTTGGCAC	50.0	50.0	58.0	58.0	1234 bp	1.0	80.6
ycf1_p2	GTACGACGTTGATTGGCTCT	CAGTTTGGGGAATGGAAGCT	50.0	50.0	58.0	58.1	1144 bp	1.0	80.3
ycf1_p3	GTACGACGTTGATTGGCTCT	CTGTCTGAAACCTTGGCACA	50.0	50.0	58.0	58.3	1233 bp	1.0	78.3
ycf1_p4	GTACGACGTTGATTGGCTCT	ACAGTTTGGGGAATGGAAGC	50.0	50.0	58.0	58.4	1145 bp	1.0	77.9
ycf1_p5	GTACGACGTTGATTGGCTCT	TTCTGTCTGAAACCTTGGCA	50.0	45.0	58.0	57.3	1235 bp	1.0	75.0