Hotspot

rpl16

LSC · 84264-85706 · 1443 bp

Back to Caiophora

Pi 0.00763

MarkerSeek score 63.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1443
variable_sites: 11
pi: 0.00763
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TTGTTTCAGTCCCAAGACCT	TGACCATTTGATACAATTGCT	45.0	33.3	56.6	53.8	1505 bp	1.0	40.6
rpl16_p2	TTGTTTCAGTCCCAAGACCT	TTGACCATTTGATACAATTGCT	45.0	31.8	56.6	54.6	1506 bp	1.0	40.6
rpl16_p3	TTTGTTTCAGTCCCAAGACCT	TGACCATTTGATACAATTGCT	42.9	33.3	57.3	53.8	1506 bp	1.0	40.6
rpl16_p4	TTTGTTTCAGTCCCAAGACCT	TTGACCATTTGATACAATTGCT	42.9	31.8	57.3	54.6	1507 bp	1.0	40.6
rpl16_p5	TTGTTTCAGTCCCAAGACCT	CAATTGACCATTTGATACAATTGCT	45.0	32.0	56.6	57.2	1509 bp	1.0	40.6