Hotspot

psbC

LSC · 35944-37365 · 1422 bp

Back to Caiophora

Pi 0.00141
MarkerSeek score 47.50
Primer available yes
Label psbC

Feature Metadata

label_name
psbC
feature_type
gene
region
LSC
length_bp
1422
variable_sites
2
pi
0.00141
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC_p1 TCGTTTCTCAGGAAATCCGC TGGATTCCTATTTCAAACATTGGAC 50.0 36.0 58.3 57.8 1569 bp 1.0 40.1
psbC_p2 GCAGCGGAAGATCCTGAATT TGGATTCCTATTTCAAACATTGGAC 50.0 36.0 58.3 57.8 1549 bp 1.0 40.1
psbC_p3 AGGTATTCGTGCTTGGATGG TGGATTCCTATTTCAAACATTGGAC 50.0 36.0 57.7 57.8 1493 bp 1.0 40.1
psbC_p4 TCGTTTCTCAGGAAATCCGC TCAAACATTGGACTTACTGTC 50.0 38.1 58.3 53.8 1557 bp 1.0 40.1
psbC_p5 GCAGCGGAAGATCCTGAATT TCAAACATTGGACTTACTGTC 50.0 38.1 58.3 53.8 1537 bp 1.0 40.1