Hotspot

ndhF

SSC · 111958-114204 · 2247 bp

Back to Cadaba

Pi 0.0107
MarkerSeek score 55.80
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2247
variable_sites
24
pi
0.0107
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 GGATGATGAATTCCACGTTCG AGAAACCAGTGAATCAGAAA 47.6 35.0 57.4 52.3 2384 bp 1.0 40.8
ndhF_p2 GGATGATGAATTCCACGTTCG AGATAGAAACCAGTGAATCAGA 47.6 36.4 57.4 54.3 2388 bp 1.0 40.8
ndhF_p3 GGATGATGAATTCCACGTTCG AAGATAGAAACCAGTGAATCAGA 47.6 34.8 57.4 55.1 2389 bp 1.0 40.8
ndhF_p4 GGATGATGAATTCCACGTTCG AGATAGAAACCAGTGAATCAGAA 47.6 34.8 57.4 55.1 2388 bp 1.0 40.8
ndhF_p5 GGATGATGAATTCCACGTTCG AGAAACCAGTGAATCAGAAAT 47.6 33.3 57.4 53.0 2384 bp 1.0 40.8