Hotspot

ndhA

SSC · 121578-123746 · 2169 bp

Back to Cadaba

Pi 0.00925
MarkerSeek score 59.60
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2169
variable_sites
20
pi
0.00925
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 CCCGGTTATCATAGGAAGCA GCCCACCGGGTTTTATTAAT 50.0 45.0 56.8 56.1 2389-2392 bp 1.0 55.5
ndhA_p2 ACCCGGTTATCATAGGAAGC GCCCACCGGGTTTTATTAAT 50.0 45.0 56.8 56.1 2390-2393 bp 1.0 55.5
ndhA_p3 CCCGGTTATCATAGGAAGCA TGACGATACTCGGTAGCATAGA 50.0 45.5 56.8 58.0 2320-2323 bp 1.0 55.1
ndhA_p4 ACCCGGTTATCATAGGAAGC TGACGATACTCGGTAGCATAGA 50.0 45.5 56.8 58.0 2321-2324 bp 1.0 55.1
ndhA_p5 CCCGGTTATCATAGGAAGCA ATGACGATACTCGGTAGCAT 50.0 45.0 56.8 55.9 2321-2324 bp 1.0 54.7