Hotspot

ccsA-ndhD

SSC · 117200-117486 · 287 bp

Back to Cadaba

Pi 0.0559
MarkerSeek score 75.30
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
287
variable_sites
16
pi
0.0559
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GCAATTGTGGCTTCGATAGG ACTAGCAAGTGACAAGGTCG 50.0 50.0 57.2 57.6 444-446 bp 1.0 73.4
ccsA-ndhD_p2 GCAATTGTGGCTTCGATAGG AGCAAGTGACAAGGTCGAAT 50.0 45.0 57.2 57.4 441-443 bp 1.0 72.6
ccsA-ndhD_p3 GCAATTGTGGCTTCGATAGG CTAGCAAGTGACAAGGTCGA 50.0 50.0 57.2 57.3 443-445 bp 1.0 71.2
ccsA-ndhD_p4 GCAATTGTGGCTTCGATAGG GCAAGTGACAAGGTCGAATC 50.0 50.0 57.2 57.1 440-442 bp 1.0 69.9
ccsA-ndhD_p5 CTGCAATTGTGGCTTCGATAG ACTAGCAAGTGACAAGGTCG 47.6 50.0 57.7 57.6 446-448 bp 1.0 68.9