Hotspot

rps8-rpl14

LSC · 81755-82013 · 259 bp

Back to Cadaba

Pi 0.0207
MarkerSeek score 67.80
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
259
variable_sites
5
pi
0.0207
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 ATCCGAACCGTTGCTTTTCT CCCACGGGAATTGAGACAAT 45.0 50.0 58.1 57.9 382-400 bp 1.0 82.8
rps8-rpl14_p2 ATCCGAACCGTTGCTTTTCT GAGTTTTTGGAGCGATCCCA 45.0 50.0 58.1 58.2 398-416 bp 1.0 82.2
rps8-rpl14_p3 TCGGTGATGTTAGTCGATGC CCCACGGGAATTGAGACAAT 50.0 50.0 57.8 57.9 403-421 bp 1.0 81.8
rps8-rpl14_p4 CGGTGATGTTAGTCGATGCT CCCACGGGAATTGAGACAAT 50.0 50.0 57.8 57.9 402-420 bp 1.0 81.8
rps8-rpl14_p5 ATCCGAACCGTTGCTTTTCT AACTCGAGTTTTTGGAGCGA 45.0 45.0 58.1 57.8 403-421 bp 1.0 81.7