Hotspot

trnW-CCA-trnP-UGG

LSC · 67443-67634 · 192 bp

Back to Cadaba

Pi 0.0365
MarkerSeek score 73.50
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
192
variable_sites
7
pi
0.0365
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GTTTTGGAGACCCACGTTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 271-273 bp 1.0 81.0
trnW-CCA-trnP-UGG_p2 GTTTTGGAGACCCACGTTCT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 260-262 bp 1.0 81.0
trnW-CCA-trnP-UGG_p3 GTTTTGGAGACCCACGTTCT CACGGGTTCAAATCCTGTCA 50.0 50.0 58.0 58.1 258-260 bp 1.0 81.0
trnW-CCA-trnP-UGG_p4 GTTTTGGAGACCCACGTTCT TGTCACGGGTTCAAATCCTG 50.0 50.0 58.0 58.1 261-263 bp 1.0 81.0
trnW-CCA-trnP-UGG_p5 GTTTTGGAGACCCACGTTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 272-274 bp 1.0 80.9