Hotspot

petN-psbM

LSC · 29082-29724 · 643 bp

Back to Cadaba

Pi 0.0202
MarkerSeek score 65.10
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
643
variable_sites
13
pi
0.0202
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCATGGGCT GCACTCTTCATTCTCGTTCCT 50.0 47.6 58.2 58.0 797-798 bp 1.0 71.3
petN-psbM_p2 TAGTAAGTCTCGCATGGGCT ACTGCACTCTTCATTCTCGT 50.0 45.0 58.2 56.9 800-801 bp 1.0 70.7
petN-psbM_p3 ATGGGGAAGAAGTGGACTCT GCACTCTTCATTCTCGTTCCT 50.0 47.6 57.6 58.0 736-737 bp 1.0 70.4
petN-psbM_p4 CCCTCGTAGTATGGGGAAGA GCACTCTTCATTCTCGTTCCT 55.0 47.6 57.6 58.0 746-747 bp 1.0 70.2
petN-psbM_p5 TCCCTCGTAGTATGGGGAAG GCACTCTTCATTCTCGTTCCT 55.0 47.6 57.6 58.0 747-748 bp 1.0 70.2