Hotspot

atpF-atpH

LSC · 13075-13592 · 518 bp

Pi 0.0178

MarkerSeek score 69.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 518
variable_sites: 9
pi: 0.0178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	627-636 bp	1.0	78.4
atpF-atpH_p2	CCAGTGACCCAAGTAAACGA	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.5	57.6	600-609 bp	1.0	75.1
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TTTATGGCCTGGTTGTAGCA	50.0	45.0	57.9	57.1	631-640 bp	1.0	74.6
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GGCCTGGTTGTAGCATTAGC	50.0	55.0	57.9	59.0	626-635 bp	1.0	73.9
atpF-atpH_p5	GGCCAGTGACCCAAGTAAAC	TGGCCTGGTTGTAGCATTAG	55.0	50.0	58.8	57.6	602-611 bp	1.0	73.4