Hotspot

trnS-CGA-trnR-UCU

LSC · 9681-9885 · 205 bp

Back to Cadaba

Pi 0.0406
MarkerSeek score 73.00
Primer available yes
Label trnS-CGA-trnR-UCU

Feature Metadata

label_name
trnS-CGA-trnR-UCU
feature_type
igs
region
LSC
length_bp
205
variable_sites
8
pi
0.0406
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-CGA-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 284-286 bp 1.0 67.8
trnS-CGA-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.1 56.9 287-289 bp 1.0 67.6
trnS-CGA-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 290-292 bp 1.0 67.1
trnS-CGA-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.1 57.9 293-295 bp 1.0 66.8
trnS-CGA-trnR-UCU_p5 CCCTAGCCTTCCAAGCTAAC AGAAGACCTCTGTCCTATCCA 55.0 47.6 57.7 56.9 288-290 bp 1.0 65.3