Hotspot

trnS-GCU-trnS-CGA

LSC · 8292-8889 · 598 bp

Back to Cadaba

Pi 0.0118
MarkerSeek score 64.50
Primer available yes
Label trnS-GCU-trnS-CGA

Feature Metadata

label_name
trnS-GCU-trnS-CGA
feature_type
igs
region
LSC
length_bp
598
variable_sites
7
pi
0.0118
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnS-CGA_p1 CAATCCGACGCTTTAGTCCA TGAATCAAACCGAGAGACCC 50.0 50.0 58.0 57.2 712-761 bp 1.0 75.1
trnS-GCU-trnS-CGA_p2 AATCCGACGCTTTAGTCCAC TGAATCAAACCGAGAGACCC 50.0 50.0 58.0 57.2 711-760 bp 1.0 75.0
trnS-GCU-trnS-CGA_p3 ACGGAAAGAGAGGGATTCGA TGAATCAAACCGAGAGACCC 50.0 50.0 58.2 57.2 766-815 bp 1.0 73.8
trnS-GCU-trnS-CGA_p4 CAATCCGACGCTTTAGTCCA GAATCAAACCGAGAGACCCT 50.0 50.0 58.0 56.9 711-760 bp 1.0 72.7
trnS-GCU-trnS-CGA_p5 AATCCGACGCTTTAGTCCAC GAATCAAACCGAGAGACCCT 50.0 50.0 58.0 56.9 710-759 bp 1.0 72.7