Hotspot

trnK-UUU-rps16

LSC · 4439-5402 · 964 bp

Back to Cadaba

Pi 0.0189
MarkerSeek score 71.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
964
variable_sites
18
pi
0.0189
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AACTCTACCGCAGATCTGGA AAAAAGGCGCTCAACCTACA 50.0 45.0 57.9 58.0 1161-1166 bp 1.0 80.8
trnK-UUU-rps16_p2 AACTCTACCGCAGATCTGGA AGAAGGCAGGGGTTTTTACG 50.0 50.0 57.9 58.1 1119-1124 bp 1.0 80.4
trnK-UUU-rps16_p3 AACTCTACCGCAGATCTGGA CGCTCAACCTACAGGAACAG 50.0 55.0 57.9 58.3 1154-1159 bp 1.0 78.8
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG AAAAAGGCGCTCAACCTACA 50.0 45.0 58.6 58.0 1104-1109 bp 1.0 77.6
trnK-UUU-rps16_p5 AACTCTACCGCAGATCTGGA GGCAGGGGTTTTTACGGAAT 50.0 50.0 57.9 58.4 1115-1120 bp 1.0 77.5