Hotspot

ycf1

SSC · 128625-134288 · 5664 bp

Back to Cabomba

Pi 0.0314

MarkerSeek score 61.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5664
variable_sites: 264
pi: 0.0314
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTCTTTCTCGTCCTGGTTCC	TGTCGCAAGCCTATGTGTTT	55.0	45.0	57.9	58.1	1079-1127 bp	1.0	83.8
ycf1_p2	GGTCTTTCTCGTCCTGGTTC	TGTCGCAAGCCTATGTGTTT	55.0	45.0	57.9	58.1	1080-1128 bp	1.0	83.7
ycf1_p3	GGTCTTTCTCGTCCTGGTTC	TTGTCGCAAGCCTATGTGTT	55.0	45.0	57.9	58.1	1081-1129 bp	1.0	83.7
ycf1_p4	GTCTTTCTCGTCCTGGTTCC	TTGTCGCAAGCCTATGTGTT	55.0	45.0	57.9	58.1	1080-1128 bp	1.0	83.7
ycf1_p5	GTCTTTCTCGTCCTGGTTCC	TTTGTCGCAAGCCTATGTGT	55.0	45.0	57.9	58.1	1081-1129 bp	1.0	83.7