Hotspot

rpl14-rpl16

LSC · 86582-86717 · 136 bp

Back to Cabomba

Pi 0.0343

MarkerSeek score 72.80

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 136
variable_sites: 7
pi: 0.0343
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACATCAGTTTTCGAGCTCCG	AGAGCAGCCATGGAAATAGC	50.0	50.0	58.3	58.0	263-269 bp	1.0	79.7
rpl14-rpl16_p2	ACATCAGTTTTCGAGCTCCG	GAGCAGCCATGGAAATAGCT	50.0	50.0	58.3	58.0	262-268 bp	1.0	79.7
rpl14-rpl16_p3	ACATCAGTTTTCGAGCTCCG	GCACGCAAAATGCCTATACG	50.0	50.0	58.3	58.3	242-248 bp	1.0	77.9
rpl14-rpl16_p4	GCTGTTGTCCGCTACATTCA	AGAGCAGCCATGGAAATAGC	50.0	50.0	58.6	58.0	244-250 bp	1.0	77.6
rpl14-rpl16_p5	GCTGTTGTCCGCTACATTCA	GAGCAGCCATGGAAATAGCT	50.0	50.0	58.6	58.0	243-249 bp	1.0	77.6