Hotspot

rps16-trnQ-TTG

LSC · 6162-7756 · 1595 bp

Back to Cabomba

Pi 0.0309

MarkerSeek score 77.00

Primer available yes

Label rps16-trnQ-TTG

Feature Metadata

label_name: rps16-trnQ-TTG
feature_type: igs
region: LSC
length_bp: 1595
variable_sites: 67
pi: 0.0309
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-TTG_p1	AATCCACAGCCGATCATGTC	ACGGGTTTTGGTCCTGTTAC	50.0	50.0	58.0	58.0	1646-1715 bp	1.0	86.6
rps16-trnQ-TTG_p2	GTCATTCAAGTCGCACGTTG	ACGGGTTTTGGTCCTGTTAC	50.0	50.0	58.1	58.0	1629-1698 bp	1.0	86.5
rps16-trnQ-TTG_p3	AATCCACAGCCGATCATGTC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1623-1692 bp	1.0	86.3
rps16-trnQ-TTG_p4	ACGTTGCCTTCTACCACATC	ACGGGTTTTGGTCCTGTTAC	50.0	50.0	57.9	58.0	1615-1684 bp	1.0	86.3
rps16-trnQ-TTG_p5	GTTGCCTTCTACCACATCGT	ACGGGTTTTGGTCCTGTTAC	50.0	50.0	57.9	58.0	1613-1682 bp	1.0	86.3