Hotspot

rps16

LSC · 4864-5996 · 1133 bp

Back to Byrsonima

Pi 0.00177

MarkerSeek score 52.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1133
variable_sites: 2
pi: 0.00177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGAGTTATCCACACCCTCGT	TCCGTGAACCAATGACTATTCA	50.0	40.9	58.1	57.5	1244-1248 bp	1.0	59.2
rps16_p2	CCACACCCTCGTAGTGTACT	TCCGTGAACCAATGACTATTCA	55.0	40.9	58.5	57.5	1236-1240 bp	1.0	56.1
rps16_p3	TCCACACCCTCGTAGTGTAC	TCCGTGAACCAATGACTATTCA	55.0	40.9	58.5	57.5	1237-1241 bp	1.0	56.1
rps16_p4	ATCCACACCCTCGTAGTGTA	TCCGTGAACCAATGACTATTCA	50.0	40.9	57.5	57.5	1238-1242 bp	1.0	55.6
rps16_p5	TGAGTTATCCACACCCTCGT	CCGTGAACCAATGACTATTCA	50.0	42.9	58.1	56.0	1243-1247 bp	1.0	55.4