Hotspot

rps11

LSC · 83666-84082 · 417 bp

Back to Byrsonima

Pi 0.00964

MarkerSeek score 46.50

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: LSC
length_bp: 417
variable_sites: 4
pi: 0.00964
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	TTCGTGTGCTATGTTCTCGG	GGCGGAATGGGGATTGTAAT	50.0	50.0	58.0	58.0	1432-1445 bp	1.0	81.2
rps11_p2	GCACAAATCCATTGGTTCCG	TCCGAGACTTCCCTTTCTGT	50.0	50.0	58.0	58.0	1535-1548 bp	1.0	81.1
rps11_p3	GCACAAATCCATTGGTTCCG	TGTCCGAGACTTCCCTTTCT	50.0	50.0	58.0	58.0	1537-1550 bp	1.0	81.1
rps11_p4	GCACAAATCCATTGGTTCCG	TCATTCCCCTCCACATTCCT	50.0	50.0	58.0	58.0	1482-1495 bp	1.0	81.1
rps11_p5	GCACAAATCCATTGGTTCCG	GGCGGAATGGGGATTGTAAT	50.0	50.0	58.0	58.0	1651-1664 bp	1.0	81.0