Hotspot

ccsA-ndhD

SSC · 120277-120514 · 238 bp

Back to Byrsonima

Pi 0.042
MarkerSeek score 71.80
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
238
variable_sites
10
pi
0.042
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGTCGCTTCTATTGGCTTTCT CAGTTGACAAGGTCGAAGCT 42.9 50.0 57.7 58.1 382-387 bp 1.0 70.4
ccsA-ndhD_p2 TGTCGCTTCTATTGGCTTTCT TCAGTTGACAAGGTCGAAGC 42.9 50.0 57.7 58.1 383-388 bp 1.0 70.3
ccsA-ndhD_p3 TCATTCTGCAATTGTCGCTTC CAGTTGACAAGGTCGAAGCT 42.9 50.0 57.5 58.1 394-399 bp 1.0 69.2
ccsA-ndhD_p4 TCATTCTGCAATTGTCGCTTC TCAGTTGACAAGGTCGAAGC 42.9 50.0 57.5 58.1 395-400 bp 1.0 69.1
ccsA-ndhD_p5 CATTCTGCAATTGTCGCTTCT CAGTTGACAAGGTCGAAGCT 42.9 50.0 57.5 58.1 393-398 bp 1.0 69.1