Hotspot

rpl32-trnL

SSC · 118673-119098 · 426 bp

Back to Byrsonima

Pi 0.00939

MarkerSeek score 56.30

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 426
variable_sites: 4
pi: 0.00939
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GAAAGGGGATATTAGGCGGC	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.2	58.0	517 bp	1.0	79.1
rpl32-trnL_p2	GAAAGGGGATATTAGGCGGC	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.2	58.0	518 bp	1.0	79.1
rpl32-trnL_p3	GAAAGGGGATATTAGGCGGC	TCGAACCGAGATGCTTTAGC	55.0	50.0	58.2	58.1	553 bp	1.0	78.5
rpl32-trnL_p4	ATTAGGCGGCGTTAAAAGCT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.3	58.0	507 bp	1.0	78.5
rpl32-trnL_p5	ATTAGGCGGCGTTAAAAGCT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.3	58.0	508 bp	1.0	78.5