Hotspot

psaJ-rpl33

LSC · 71515-72033 · 519 bp

Back to Byrsonima

Pi 0.0058

MarkerSeek score 60.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 519
variable_sites: 3
pi: 0.0058
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CGTGGCACCGGTAATAAGTA	ATAACCCTTACGCGGACATC	50.0	50.0	57.4	57.5	669-671 bp	1.0	74.2
psaJ-rpl33_p2	CGTGGCACCGGTAATAAGTA	TAACCCTTACGCGGACATCT	50.0	50.0	57.4	58.5	668-670 bp	1.0	74.1
psaJ-rpl33_p3	CGTGGCACCGGTAATAAGTA	CCCTTACGCGGACATCTTTC	50.0	55.0	57.4	58.7	665-667 bp	1.0	72.6
psaJ-rpl33_p4	CGTGGCACCGGTAATAAGTA	CCTTACGCGGACATCTTTCC	50.0	55.0	57.4	58.7	664-666 bp	1.0	72.6
psaJ-rpl33_p5	CGTGGCACCGGTAATAAGTA	CTTACGCGGACATCTTTCCC	50.0	55.0	57.4	58.7	663-665 bp	1.0	72.6