Hotspot

psaA-ycf3

LSC · 44997-45825 · 829 bp

Back to Byrsonima

Pi 0.00124

MarkerSeek score 62.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 1
pi: 0.00124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	985-1007 bp	1.0	80.6
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	967-989 bp	1.0	80.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	GCGCTTACCCCTGGAAATTA	50.0	50.0	57.9	58.0	953-975 bp	1.0	80.6
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TATAGCGCTTACCCCTGGAA	50.0	50.0	57.9	57.9	957-979 bp	1.0	80.2
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	927-949 bp	1.0	80.2