Hotspot

psbZ-trnG

LSC · 39088-39636 · 549 bp

Back to Byrsonima

Pi 0.011

MarkerSeek score 61.40

Primer available yes

Label psbZ-trnG

Feature Metadata

label_name: psbZ-trnG
feature_type: igs
region: LSC
length_bp: 549
variable_sites: 6
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	703-866 bp	1.0	79.1
psbZ-trnG_p2	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	699-862 bp	1.0	77.5
psbZ-trnG_p3	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	702-865 bp	1.0	76.4
psbZ-trnG_p4	TCTCCTGATGGTTGGTCGAG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.8	57.8	701-864 bp	1.0	72.7
psbZ-trnG_p5	CTTCTCCTGATGGTTGGTCG	CGGATAGCGGGAATCGAAC	55.0	57.9	58.0	58.2	725-888 bp	1.0	71.0